SMT Columns

Separation Methods Technologies, Inc. (SMT) manufactures a full variety of capillary, UPLC, UHPLC, HPLC, LPLC, SFC, GPC, SEC and other chromatography columns and resins for all methods development, validation, and scale up needs.

SMT has built a reputation as a manufacturer of the highest quality bulk packing material. We offer a wide selection of silica sizes of various pore dimensions, for various chromatographic applications. The Horizontal Polymerized Bonded Phase packing material offers the benefits of maximized silica life, which reduces manufacturing down time, waste disposal, and increased overall efficiency.

The following chart has been provided as a guide to selecting HPLC columns which meet the specifications set forth in the United States Pharmacopeia [USP], which provides guidelines for the separation of related compounds.

It is important to note that, in many cases, USP column specifications are so broad that several (or many) column types actually meet the basic specications. For example, L1 specification calls for a column consisting of silica packing material, 5 or 10µm in diameter, bonded with octadecyl (C18) silane. However, a limited number of available C18 columns will actually perform the desired separation.

SMT offers a variety of columns for each category. These columns are representative of the wide range of selectivities available for each bonded phase. The packing materials vary in particle size, pore size, surface area, carbon load, hydrophobicity, bonded phase coverage or density, and other characteristics. Refer to the catalog for description and characteristics of a specific column of interest.

United States Pharmacopeia [USP]

Column	Description	Column(s)
L1	Octadecylsilane chemically bonded to porous or non-porous silica or ceramic microparticles, 1.5 to 10µm in diameter	OD ODL Elite C18 Symplex C18 Supreme C18 AquSep C18
L2	Octadecylsilane (C18) chemically bonded to silica gel of a controlled surface porosity, 30 to 50 µm in diameter	BOD Bulk Packings BODL Bulk Packings
L3	Porous silica particles, 1.5 to 10µm in diameter	S
L4	Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50µm in diameter	BS Bulk Packings
L7	Octylsilane chemically bonded to totally or superficially porous silica particles, 1.5 to 10µm in diameter	O OL Elite C8
L8	Aminopropylsilane chemically bonded to totally porous silica gel support, 1.5 to 10µm in diameter	AP (NH2)
L9	Porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10µm in diameter	SCX
L10	Nitrile groups chemically bonded to porous silica particles, 1.5 to 10µm in diameter	CP (CN)
L11	Phenyl groups chemically bonded to porous silica particles, 1.5 to 10µm in diameter	Phen1 Phen2
L12	A strong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core, 30 to 50µm in diameter	BSAX Bulk Packings
L13	Trimethylsilane chemically bonded to porous silica particles, 3 to 10µm in diameter	MEB1 C1
L14	Silica gel having a chemicallly bonded, strongly basic quaternary ammonium anion-exchange coating, 5 to 10µm in diameter	SAX
L15	Hexylsilane chemically bonded to totally porous silica particles, 3 to 10µm in diameter	Hex C6
L16	Dimethylsilane chemically bonded to porous silica particles, 5 to 10µm in diameter	MEB2-DM C2
L18	Amino and cyano groups chemically bonded to porous silica particles, 3 to 10µm in diameter	AP-CP
L20	Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to 10µm in diameter	DIOL2
L26	Butylsilane chemically bonded to totally porous silica particles, 1.5 to 10µm in diameter	MEB4 C4
L27	Porous silica particles, 30 to 50µm in diameter	BS Bulk Packings
L30	Ethyl silane chemically bonded to 3-10µm porous silica	MEB2 C2
L40	Cellulose tris-3,5-dimethylphenylcarbamate bonded on porous silica, 5-20µm	ChiralSep1
L41	Immobilised alpha-1-acid glycoprotein on spherical silica particles, 5µm in diameter	ChiralSepAGP
L42	Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5µm in diameter	ODO
L43	Pentafluorophenyl groups chemically bonded to silica particles 5 to 10µm in diameter	C6F5
L44	Spherical silica substrate that has been bonded with a cationic exchanger, sulphonic L44 acid functionality in addition to a conventional reversed phase C8 functionality	SCX-O
L52	Strong Cation Exchange resin made of porous silica with sulfopropyl groups, 5 to 10µm in diameter	SCX
L56	Propyl silane chemically bonded to totally porous silica particles, 3 to 10µm in diameter	Pro
L57	Chiral recognition protein, ovomucoid, chemically bonded to silica particles, about 5 µm in diameter, with pore size of 120Å	CIB Ovomucoid
L59	Packing having the capacity to separate proteins by molecular weight over the range of 10 to 500 kDa. It is spherical (10 µm), silica-based, and processed to provide hydrophilic characteristics and pH stability	SMTgel Diol
L60	Spherical porous silica gel, 3 or 5 µm diameter, the surface of which has been covalently modified with alkyl amide groups	AquSep2
L62	C30 silane bonded phase on a fully porous spherical silica, 5-10µm in diameter	TA
L63	Glycopeptide teicoplanin linked through multiple covalent bonds to spherical silica	CIB TE
L70	Cellulose tris(phenyl carbamate) coated on 5-10µm porous silica	ChiralSep2
L80	Cellulose tris(4-methylbenzoate)-coated, porous, spherical, silica particles, 5 to 20 µm in diameter	ChiralSep3
L85	A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and weak cation-exchange (carboxyl groups) functional groups chemically bonded to porous or non-porous particles, 1.0 to 50 µm in diameter	WCX-O
L93	Cellulose tris(3,5-dimethylpheylcarbamate) reversed phase chiral stationary phase coated on 3 or 5 µm silica gel particles	ChiralSep1
L107	Cellulose tris(4-methylbenzoate)-coated porous spherical particles, 3 to 5 µm in diameter	ChiralSep3
Unclassified	Dodecyl silane bonded on 5-10µm porous silica	DD
Unclassified	Proprietary strong cation exchange groups on 5-50µm Porous silica	MetalSep