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Aminex® Carbohydrate Analysis Columns

Aminex HPLC columns are packed with a polymer-based matrix (polystyrene divinylbenzene). They separate compounds using the ion-moderated partition chromatography technique. Aminex carbohydrate analysis columns separate compounds using a combination of size exclusion and ligand exchange mechanisms:

  • In oligosaccharide separations, size exclusion is the primary mechanism. Low crosslinked resins allow carbohydrates to penetrate, and oligosaccharides separate by size
  • For monosaccharide separations, ligand exchange is the primary mechanism and involves the binding of hydroxyl groups of the sugars with the fixed counterion of the resin. Ligand exchange is affected by the nature of the counterion (Pb2+, Ca2+, etc.) and by the spatial orientation of the carbohydrate's hydroxyl groups

Aminex columns allow the use of simple isocratic methods, eluting with water or dilute acid. There is minimal sample preparation, usually just filtering through a 0.45 µm filter with no derivatization necessary. Aminex media have a high pressure stability, wide pH stability, and high column efficiency and selectivity.

Aminex columns are an industry standard for the analysis of carbohydrates. Bio-Rad offers a complete line of carbohydrate analysis columns optimized to provide high selectivity for a specific carbohydrate or carbohydrate class. Research length (300 mm) columns provide high-resolution separations of complex carbohydrate mixtures in approximately 20 minutes, while the shorter columns provide efficient separations of specific carbohydrates in 3–5 minutes. Use our selection guide to choose the column that fits your application needs.

Aminex HPX-87C Column
This calcium-form column is the column of choice for most general sweetener analyses. It is optimized for analyzing monosaccharides and also provides class separation of di-, tri-, and tetrasaccharides. The 300 x 7.8 mm column is used primarily for the quantitation of glucose and fructose in high fructose corn syrup and for general monosaccharide analysis. The 250 x 4.0 mm column is appropriate for sugar alcohol separations.

Aminex HPX-87P Column
This lead-form, 300 x 7.8 mm column is tailored for the separation of cellulose-derived monosaccharides. It is used in analyses of pentoses and hexoses in wood products, especially cellobiose, glucose, xylose, galactose, arabinose, and mannose. It also provides excellent resolution of sucrose, lactose, and fructose in dairy products.

Fast Carbohydrate Analysis Column
This lead-form, 100 x 7.8 mm column is tailored for extremely fast separations of specific carbohydrates in samples where only certain components are of interest. It is optimized for 5-minute analyses of sucrose, glucose, galactose, and fructose.

Aminex HPX-87H Column
This hydrogen-form, 300 x 7.8 mm column is used for analysis of carbohydrates in solution with carboxylic acids, volatile fatty acids, short chain fatty acids, alcohols, ketones, and many neutral metabolic by-products. Most often used for organic acid analysis, this column is also useful for fermentation monitoring, biological fluid analysis, and acetylated amino sugar separations.

Aminex HPX-87N Column
This sodium-form, 300 x 7.8 mm column is optimized for the analysis of sugars in samples with high salt concentrations, such as beet sugars. It is compatible with salts, so there is no need to desalt samples before analysis.

Aminex HPX-87K Column
This potassium-form, 300 x 7.8 mm column is optimized for the analysis of mono-, di-, and trisaccharides in samples such as corn syrup and brewing wort. It exhibits good separations of glucose, maltose, and maltotriose.

Aminex HPX-42A Column
This silver-form, 300 x 7.8 mm column provides fast, high-resolution oligosaccharide analysis. It separates oligosaccharides to D-11 in approximately 25 minutes.

Aminex HPX-42C Column
This calcium-form, 300 x 7.8 mm column is optimized for analysis of mono- and disaccharides in starch hydrolysates. It also provides excellent resolution of oligosaccharides as large as D-10.

In-Line Carbohydrate Deashing System
This in-line deashing system protects the Aminex carbohydrate columns from contamination by removing all inorganic salts from the sample, allowing only neutral carbohydrates to pass to the analytical column. The system consists of two Micro-Guard cartridges, one packed with anion and the other with cation exchange resin. Installed in series in front of Bio-Rad analytical columns, this system offers convenient and highly efficient in-line purification of sugar samples.

 

Aminex HPX-87N Column

1250143
Pkg of 1, 300 x 7.8 mm, prepacked HPLC carbohydrate analysis column, sodium form, 9 µm particle size, 8% cross linkage, pH range 5–9

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Aminex HPX-87K Column

1250142
Pkg of 1, 300 x 7.8 mm, prepacked HPLC carbohydrate analysis column, potassium form, 9 µm particle size, 8% cross linkage, pH range 5–9

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Aminex HPX-87C Column

1250095
Pkg of 1, 300 x 7.8 mm, prepacked HPLC carbohydrate analysis column, calcium form, 9 µm particle size, 8% cross linkage, pH range 5–9

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Aminex HPX-87C Column

1250094
Pkg of 1, 250 x 4.0 mm, prepacked HPLC carbohydrate analysis column, calcium form, 9 µm particle size, 8% cross linkage, pH range 5–9

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Aminex HPX-87P Column

1250098
Pkg of 1, 300 x 7.8 mm, prepacked HPLC carbohydrate analysis column, hydrogen form, 9 µm particle size, 8% cross linkage, pH range 5–9

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Aminex HPX-87H Column

1250140
Pkg of 1, 300 x 7.8 mm, prepacked HPLC carbohydrate analysis column, hydrogen form, 9 µm particle size, 8% cross linkage, pH range 1–3

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Aminex HPX-42C Column

1250096
Pkg of 1, 300 x 7.8 mm, prepacked HPLC carbohydrate analysis column, calcium form, 25 µm particle size, 4% cross linkage, pH range 5–9

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Aminex HPX-42A Column

1250097
Pkg of 1, 300 x 7.8 mm, prepacked HPLC carbohydrate analysis column, silver form, 25 µm particle size, 4% cross linkage, pH range 6–8

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Fast Carbohydrate Column

1250105
Pkg of 1, 100 x 7.8 mm, prepacked HPLC carbohydrate analysis column, lead form, 9 µm particle size, 8% cross linkage, pH range 5–9

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Carbohydrate Analysis Kit

1250233
Application kit for food analysis, calcium form, 9 μm particle size, 8% cross linkage, pH range 5–9, includes guard column

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Aminex Organic Acid and Alcohol Analysis Columns

 

Aminex HPLC columns are packed with a polymer-based matrix (polystyrene divinylbenzene). They separate compounds using the ion-moderated partition chromatography technique.

HPLC separations using Aminex columns employ the mechanisms of ion exclusion, ion exchange, ligand exchange, size exclusion, reversed phase, and normal phase partitioning. These multiple modes of interaction offer a unique ability to separate compounds. Several Aminex columns are available for small acidic and polar molecule analysis.

Aminex columns allow the use of simple isocratic methods, eluting with water or dilute acid. There is minimal sample preparation, usually just filtering through a 0.45 μm filter with no derivatization necessary. Aminex media have a high pressure stability, wide pH stability, and high column efficiency and selectivity.

Aminex columns are an industry standard for the analysis of organic acids and alcohols.Bio-Rad offers organic acid columns for simple, yet specific methods of food and beverage analysis. Use our selection guide to choose the column that fits your application needs.

Aminex HPX-87H Column
This hydrogen-form, 300 x 7.8 mm column performs most analyses in about 20 minutes, with sensitivity to the nanogram level. A simple isocratic elution with slightly acidified water is all that it takes to analyze organic acids in most samples, and filtration is the only sample preparation required before injection. Separations can be performed from ambient temperature to 60°C at flow rates of 0.4–1.0 ml/min. When many compounds in a formulation must be analyzed, or when high-resolution separations are required, this is the column of choice.

Fast Acid Analysis Column
This hydrogen-form, 100 x 7.8 mm column is optimized for the analysis of alcohols, glycols, and hydrophobic organic acids. Typically, analyses can be shortened 4-fold over those obtained with research-length columns. Because the fast acid column is shorter, components elute as taller, narrower peaks. Detection limits are improved, and smaller sample loads can be used. This column provides fast separations of specific organic acids in samples where only certain components are of interest. With these columns, analyses can be completed in 3–5 minutes. As with the Aminex HPX-87H column, dilute sulfuric acid is the only eluent used, and filtering is generally the only sample preparation required.

Fermentation Monitoring Column
This hydrogen-form, 150 x 7.8 mm column is optimized to resolve maltotriose, maltose, glucose, and fructose while also resolving acids and alcohols. When analysis of sugars in a fermentation broth is required, this is the column of choice. Dilute sulfuric acid is the only eluent used, and filtering is generally the only sample preparation required.

Aminex HPX-87C Column
This calcium-form, 300 x 7.8 mm column can be fine-tuned for organic acid analysis by using an eluent that contains a mixture of water, calcium sulfate, sulfuric acid, and acetonitrile. It is also suited for analyzing organic acids in combination with carbohydrates.

 

Aminex HPX-87H Column

1250140
Pkg of 1, 300 x 7.8 mm, prepacked HPLC carbohydrate analysis column, hydrogen form, 9 µm particle size, 8% cross linkage, pH range 1–3

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Fast Acid Analysis Column

1250100
Pkg of 1, 100 x 7.8 mm, prepacked HPLC organic acid and alcohol column, hydrogen form, 9 μm particle size, 8% cross linkage, pH range 1–3

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Fermentation Monitoring Column

1250115
Pkg of 1, 150 x 7.8 mm, prepacked HPLC organic acid and alcohol column, hydrogen form, 9 μm particle size, 8% cross linkage, pH range 1–3

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Organic Acid Analysis Kit

1250234
Application kit for food analysis, hydrogen form, 9 μm particle size, 8% cross linkage, pH range 1–3, includes guard column

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